DSTL’s Approaches to Generic Sample Preparation for the Detection of Biological Agents to be Presented at Knowledge Foundations Sample Prep 2013 Conference

Boston (PRWEB) February 28, 2013

Industry leaders from around the world will convene in San Diego, CA for the Knowledge Foundations 7th Sample Prep 2013 conference which will take place at the San Diego Hilton & Resort on May 9 10. One of the many anticipated presentations will include Martin McDonnell, PhD, of the Defense Science and Technology Laboratory (DSTL) as he present Approaches to Generic Sample Preparation for the Detection of Biological Agents in the Environment

Presentation Abstract:

Approaches to Generic Sample Preparation for the Detection of Biological Agents in the Environment

Martin McDonnell, PhD, Defense Science and Technology Laboratory (DSTL)

High confidence detection of potentially hazardous pathogenic micro-organisms and protein toxins in the environment requires effective and rapid sample prep. As the consequences of a positive alert in either military or civilian settings could be severe, it is desirable that monitoring is performed using at least two very different detection technologies, including, for example immunoassay, nucleic acid or mass spectrometry-based methods. To meet this requirement, the sample prep should ideally be generic, with an output sample suitable for a broad range of sensor types. When added to the high variability of environmental backgrounds, including both biological and non-biological materials, and the need to deal with multiple sample inputs including, for example aerosols, soils, vegetation, or waste water, the development of such a system represents a significant technical challenge. In order to perform its required role, the sample prep system may perform sample maceration and agent extraction, selective background and interferent removal, agent clean-up, concentration and lysis for antigen and nucleic acid release. This requires the use of a range of processing technologies which must be capable of integration into a fully automated system capable of being used in the field by non-expert users. This presentation will describe progress in the development of such a generic integrated sample prep system in a project involving DSTL, academia and industry.

Sample Prep 2013 Program topics will include:

Robust methodologies for sample collection, (pre-)concentration, lysis, and target extraction
Critical role of sample prep in early diagnostics
Rapid sample-to-sequence techniques
Point-of-care sampling, detection and analysis
Sample preparation with micro- and nano fluidics
Robust sampling methodologies (automation, high-throughput, combinatorial approach in sample prep)
Alternative amplification techniques for sensitive sample prep
End-user prospective for biodetection and sampling technologies and devices
Alternative and disruptive approaches to sample preparation and applications
Field-ready devices: compatibility/reliability/scalability
Target enrichment and background depletion techniques
Isolation of dilute objects on cell and molecular levels
Immiscible phase purification
Novel methods of extraction from tough (non-filterable) matrices
Nucleic acid and protein based sample prep for next generation sequencing
Nanotechnology and miniaturization challenges for sample preparation
Standardization and regulatory issues in sample preparation across different applications

Distinguished Speaker Faculty:

-Charles Young, PhD, The Johns Hopkins University

-Richard Allen, PhD, Luminex Corporation

-John C. Carrano, PhD, Paratus Diagnostics, LLC

-Forest L. Rohwer, PhD, San Diego State University

-Sarah Fakih, PhD, QIAGEN GmbH, Germany

-Season Wong, PhD, AI Biosciences, Inc.

-Michael J. Heller, PhD, University of California San Diego

-Martin McDonnell, PhD, Defense Science and Technology Laboratory – DSTL Porton Down, United Kingdom

-Alexis Sauer-Budge, PhD, Fraunhofer USA

-Gregory Auner, PhD, Wayne State University

-Hafsa Korri-Youssoufi, PhD, University Paris-Sud, France

-Shan Gao, PhD, Wave 80 Biosciences, Inc.

-David Wright, Wi Medical Device Development, Inc.

-Mark W. Eshoo. PhD, Ibis Biosciences Inc., an Abbott Company

-Mark Tondra, PhD, Diagnostic Biosensors

-Patrick Broyer, PhD, bioM

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